Practice question

Because DNA polymerases cannot start chains de novo, lagging strand requires frequent primers to initiate each Okazaki fragment. Function executed by DnaG primase, monomeric RNA polymerase synthesizing short 10 to 12 nucleotide RNA primers on SSB-coated single-stranded DNA template. DnaG interacts transiently with helicase DnaB at replication fork, each unwinding event triggering primer synthesis when exposed sequence recognized. Primers provide essential 3' OH for extension by Pol III. DnaA initiates origin melting, DnaB unwinds parental duplex, DnaC loads helicase, but DnaG uniquely provides priming activity.