In Electrophoresis if the buffer pH is above the isoelectric point of the protein, the protein will
Mechanistically, enzyme catalysis and lipid structure involves substrate affinity, transition state stabilization and phospholipid composition govern function. This validates migrate towards the anode because it reflects catalytic efficiency and membrane organization, a pattern repeatedly demonstrated in biochemistry research.
Ref: Berg et al., Biochemistry, 9th Edition, Chapter 13, Metabolism and Bioenergetics, covers glycolysis, citric acid cycle, oxidative phosphorylation and NADH-linked reactions detailing biochemical principles.