Highly purified peptides Pl, P2 and P3 were subjected to MALDI mass spectral analysis. The following observations were m
Analysis of highly purified peptides pl, p2 and p3 shows P1; Met is oxidized; P2: is phosphorylated; P3: is a covalen represents established outcome. functional assays supports option B, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.
Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 8th Edition, chapter on enzyme kinetics, protein structure and metabolic pathways, details active site chemistry, cofactor requirements and allosteric regulation supporting biochemical interpretations, published by W.H. Freeman.