Practice question
Question
Highly purified peptides Pl, P2 and P3 were subjected to MALDI mass spectral analysis. The following observations were made: P1: Showed a m/z of 16 more than the expected value. P2: Showed a m/z of 80 more than the expected value. MS/MS spectra of the peptide resulted in a precursor ion with m/z 98 less than the expected m/z. P3: Showed a m/z that was double the expected value. [Note: z = + 1 for all the mass spectra.] Which one of the options given below comprises all correct interpretations?
Explanation
Analysis of highly purified peptides pl, p2 and p3 shows P1; Met is oxidized; P2: is phosphorylated; P3: is a covalen represents established outcome. functional assays supports option B, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.
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