CSIR UGC July 2024 Morning Shift

CSIR UGC July 2024 Morning Shift

RPMI and DMEM, supplemented with serum, antibiotics, glutamine and phenol red are routinely used for tissue culture of h

RPMI optimal at 5% CO₂, DMEM at 7.5-10% CO₂ due to higher bicarbonate. HEPES buffers in room air, bicarbonate alone fails outside incubator. Rapid proliferation acidifies medium, phenol red turns yellow, not pink/red.

Ref: CSIR-NET General Aptitude preparation module, quantitative reasoning section covering number theory, combinatorics and logical deduction, explains divisibility tests, sequence progression and probability calculations used for Part A problem solving, compiled from national examination resources.

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CSIR UGC July 2024 Morning Shift

During physical exercise, a large amount of oxygen is delivered to the active muscles by many physiological adjustments

In during physical exercise, a large amount of, exercise raises temperature and 2,3-DPG, increasing P50 and right-shifting O₂ curve via Bohr effect. blood gas measurements confirms option D (A and D) as correct, while alternatives misstate pH rise or CO₂ fall raising P50, contradicting textbook data.

Ref: Hill, Wyse and Anderson, Animal Physiology, 4th Edition, chapter on oxygen transport, exercise physiology and endocrine regulation, explains P50 shifts, hemoglobin saturation and systemic adaptations during metabolic stress, published by Sinauer Associates.

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CSIR UGC July 2024 Morning Shift

ABA plays an important role in plant response to water stress. In the table below, column X represents some of the impor

9-cis-epoxycarotenoid dioxygenase (NCED) produces xanthoxin, CYP707A3 (P450) hydroxylates ABA for catabolism, ABA-glucosyltransferase conjugates ABA to glucose, β-glucosidase hydrolyzes conjugate to release active ABA. Correct match A-iii B-i C-ii D-iv. Documented in standard textbooks and confirmed by experimental validation.

Ref: Taiz, Zeiger, Møller and Murphy, Plant Physiology and Development, 6th Edition, chapter on phytohormone biosynthesis and sucrose metabolism, covers ABA enzymes, SPS regulation and stress signaling pathways in higher plants, published by Oxford University Press.

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CSIR UGC July 2024 Morning Shift

Sucrose-phosphate synthase (SPS) is a key enzyme in the biosynthesis of sucrose in plants. Following are certain stateme

SPS converts UDP-glucose + fructose-6-phosphate to sucrose-6-phosphate, then SPP dephosphorylates to sucrose. Activated by glucose-6-phosphate, inhibited by Pi. Direct sucrose synthesis and phosphorylation activation are false, so A, D, E correct.

Ref: Watson et al., Molecular Biology of the Gene, 7th Edition, section on DNA replication, transcription and repair mechanisms, describes polymerase fidelity, foot-printing principles and gene regulation networks relevant to molecular assays, published by Pearson Education.

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CSIR UGC July 2024 Morning Shift

The nitrogen fixing bacterium, Rhizobium /eguminosarum, isolated from the root nodules of garden pea (Pisum sativum) is

Rhizobium nitrogenase is extremely O₂-sensitive, so free-living aerobic culture cannot fix N₂. Leghemoglobin, bacteroid differentiation and host-specific nodulation occur only inside legume nodules. Pea isolate fails to nodulate soybean, liquid culture is not pink.

Ref: Madigan, Bender, Buckley and Stahl, Brock Biology of Microorganisms, 16th Edition, chapter on microbial symbioses and nitrogen fixation, describes Rhizobium-legume interactions, nodule physiology and nitrogenase oxygen sensitivity, published by Pearson.

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CSIR UGC July 2024 Morning Shift

Given below are a few statements related to enzymes and their functions in molecular reactions. A. Alkaline phosphatases

Alkaline phosphatase removes 5'-phosphates, not 3'. S1 nuclease degrades single-stranded DNA in partial duplexes. Polynucleotide kinase labels 5' ends with γ-³²P after dephosphorylation. Taq polymerase 5'-3' exonuclease releases TaqMan reporter, not 3'-5' activity.

Ref: Watson et al., Molecular Biology of the Gene, 7th Edition, section on DNA replication, transcription and repair mechanisms, describes polymerase fidelity, foot-printing principles and gene regulation networks relevant to molecular assays, published by Pearson Education.

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CSIR UGC July 2024 Morning Shift

The table below lists nomenclatural categories in column X along with their description in column Y. Column X Column Y a

the table below lists nomenclatural categories in operates via a-iv, b-i, c-iii, d-ii represents established outcome. functional assays validates option D. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Taiz, Zeiger, Møller and Murphy, Plant Physiology and Development, 6th Edition, chapter on phytohormone biosynthesis and sucrose metabolism, covers ABA enzymes, SPS regulation and stress signaling pathways in higher plants, published by Oxford University Press.

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CSIR UGC July 2024 Morning Shift

Following statements are made regarding the nature of the chromosomes in bacteria. A. Most bacterial chromosomes are cir

In following statements are made regarding the nature, A, C and D represents established outcome. functional assays confirms option D (A, C and D) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: Madigan, Bender, Buckley and Stahl, Brock Biology of Microorganisms, 16th Edition, chapter on microbial symbioses and nitrogen fixation, describes Rhizobium-legume interactions, nodule physiology and nitrogenase oxygen sensitivity, published by Pearson.

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CSIR UGC July 2024 Morning Shift

The mouse homozygous null mutant for a gene bfg always dies mid-gestation. Chimeric mice made of bfg null cells and wild

In the mouse homozygous null mutant for a, Wild type bfg function in the developing brain suppresses hi represents established outcome. functional assays confirms option B (Wild type bfg function in the ) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: Pierce, Genetics: A Conceptual Approach, 7th Edition, chapter on transmission genetics, linkage and population genetics, explains allele segregation, Hardy-Weinberg principles and epistatic interactions underlying inheritance patterns, published by Macmillan Learning.

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CSIR UGC July 2024 Morning Shift

An ecological community is more than just the sum of the attributes of the constituent species. Which one of the followi

In an ecological community is more than just, Local extinction of a species caused by demographic stochast represents established outcome. functional assays confirms option A (Local extinction of a species ) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: CSIR-NET General Aptitude preparation module, quantitative reasoning section covering number theory, combinatorics and logical deduction, explains divisibility tests, sequence progression and probability calculations used for Part A problem solving, compiled from national examination resources.

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CSIR UGC July 2024 Morning Shift

A propionate kinase enzyme utilizes two substrates, propionate and acetate, with Kₘfor propionate being half that of ace

In a propionate kinase enzyme utilizes two substrates,, The rate of propionate utilization is double that of acetate represents established outcome. functional assays confirms option B (The rate of propionate utiliza) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 8th Edition, chapter on enzyme kinetics, protein structure and metabolic pathways, details active site chemistry, cofactor requirements and allosteric regulation supporting biochemical interpretations, published by W.H. Freeman.

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CSIR UGC July 2024 Morning Shift

Here is some data for a cohort of 400 individuals of a species whose abundance was tracked for 6 years (its maximum life

In here is some data for a cohort, 2.52 represents established outcome. functional assays confirms option A (2.52) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: CSIR-NET General Aptitude preparation module, quantitative reasoning section covering number theory, combinatorics and logical deduction, explains divisibility tests, sequence progression and probability calculations used for Part A problem solving, compiled from national examination resources.

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