Practice question

Question

Given below are a few statements related to enzymes and their functions in molecular reactions. A. Alkaline phosphatases remove 3' phosphates from DNA and RNA. B. S1 nuclease removes single-stranded regions from partially doublestranded DNA. C. 5' end-labelling of DNA molecules can be done by using polynucleotide kinase which transfers a 32P-labelled phosphate group to the 5' end of dephosphorylated DNA. D. 3'-5' exonuclease activity of Taq polymerase releases the reporter from the 3' end of Taqman probes in qPCR. Which one of the following options represents a combination of all correct statements?