You have transiently expressed a new protein (for which no antibody is available) in a cell line to establish structure
you have transiently expressed a new protein operates via Making a GFP fusion protein with this new protein represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
Ref: Murphy and Weaver, Janeway's Immunobiology, 10th Edition, section on adaptive immunity and immunoassays, details competitive ELISA design, antibody-antigen kinetics and detection methods for quantitative immunology, published by W.W. Norton.