Practice question

MCQ

Question

You have transiently expressed a new protein (for which no antibody is available) in a cell line to establish structure function relationship. Which one of the following strategies is the most straight forward way to examine the expression profile of this new protein?

Options

Choose one · Correct answer highlighted

By metabolic labelling using 35S labelled amino acids Making a GFP fusion protein with this new protein Correct lmmunoprecipitating this protein with the help of another protein for which antibody is available Running SDS-PAGE and identify the protein

Explanation

you have transiently expressed a new protein operates via Making a GFP fusion protein with this new protein represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

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