Practice question

Question

A researcher is studying the subcellular localization of a particular protein 'X' in an animal cell. The researcher performs successive centrifugation at increasing rotor speed. The researcher starts spinning the cellular homogenate at 600g for 10 min, collects the pellet, spins the supernatant at 10,000 g for 20 min, collects the pellet, spins the supernatant at 100,000g for 1 hour, collects both the pellet and the final supernatant. On subjecting various pellets and the final supernatant to Western blotting with anti-protein-X antibody, the protein X is, observed to be maximally expressed in pellet after centrifugation at 10,000 g. Based on the above observation, what will be the most likely localization of protein X.