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Primase synthesizes the short RNA primer required to initiate DNA synthesis. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.
Ref: NCERT Biology Textbook for Class XI and XII (Botany section), Chapter: Morphology and Anatomy of Flowering Plants, Topic: Plant structure and tissue systems.
Because DNA polymerases cannot start chains de novo, lagging strand requires frequent primers to initiate each Okazaki fragment. Function executed by DnaG primase, monomeric RNA polymerase synthesizing short 10 to 12 nucleotide RNA primers on SSB-coated single-stranded DNA template. DnaG interacts transiently with helicase DnaB at replication fork, each unwinding event triggering primer synthesis when exposed sequence recognized. Primers provide essential 3' OH for extension by Pol III. DnaA initiates origin melting, DnaB unwinds parental duplex, DnaC loads helicase, but DnaG uniquely provides
Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 5: DnaG primase function at bacterial replication fork
Primase activity is fundamentally RNA polymerizing, incorporating ribonucleoside triphosphates ATP, UTP, GTP, CTP complementary to DNA template without requirement for existing primer, unlike DNA polymerase. In bacteria, DnaG is monomeric RNA polymerase interacting with helicase DnaB, synthesizing ~10 nt primers for Okazaki fragments. In eukaryotes, heterotetrameric Pol α-primase combines two primase subunits Pri1/Pri2 for RNA synthesis with Pol α DNA polymerase extension of about 20 nucleotides. Despite functional similarity to RNA polymerase II that transcribes mRNA, primase is distinct gene
Ref: Frick DN, Richardson CC, Annu Rev Biochem 2001: Primase is a Type of RNA Polymerase
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