IPTG is used as an inducer in the T7 expression system for recombinant protein expression in E.coli. This is because of:
Mechanistically, enzyme catalysis and lipid structure involves substrate affinity, transition state stabilization and phospholipid composition govern function. This validates availability of special E.coli cells which have the T7 RNA polymerase gene in... because it reflects catalytic efficiency and membrane organization, a pattern repeatedly demonstrated in biochemistry research.
Ref: Berg, Tymoczko, Gatto and Stryer, Biochemistry, 9th Edition, Chapter 13, Metabolism and Bioenergetics, covers glycolysis, citric acid cycle, oxidative phosphorylation and NADH-linked reactions detailing biochemical principles of cellular energy metabolism.
