Practice question
Question
IPTG is used as an inducer in the T7 expression system for recombinant protein expression in E.coli. This is because of:
Explanation
Mechanistically, enzyme catalysis and lipid structure involves substrate affinity, transition state stabilization and phospholipid composition govern function. This validates availability of special E.coli cells which have the T7 RNA polymerase gene in... because it reflects catalytic efficiency and membrane organization, a pattern repeatedly demonstrated in biochemistry research.
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