The presence of polyadenylation signals in the wild type CRY1Ac gene from Bacillus thuringiensis prevented expression of
The principle of enzyme catalysis and lipid structure explains that substrate affinity, transition state stabilization and phospholipid composition govern function. Hence The CRY1Ac gene sequence was modified taking help of codon degeneracy fits best because it reflects catalytic efficiency and membrane organization, as documented in authoritative sources on biochemistry.
Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 7th Edition, Chapter 6, Enzyme Kinetics and Regulation, discusses Michaelis-Menten equation, transition state stabilization and allosteric control supporting enzyme function.
