Practice question
Question
The presence of polyadenylation signals in the wild type CRY1Ac gene from Bacillus thuringiensis prevented expression of appropriate amount of CRY1Ac protein in transgenic plants. What was done to overcome this problem?
Explanation
The principle of enzyme catalysis and lipid structure explains that substrate affinity, transition state stabilization and phospholipid composition govern function. Hence The CRY1Ac gene sequence was modified taking help of codon degeneracy fits best because it reflects catalytic efficiency and membrane organization, as documented in authoritative sources on biochemistry.
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