The A ₂₆₀ of a plasmid solution after 100-fold dilution is 0.2. Given that A ₂₆₀ of 1.0 represents 50 μg/ml of DNA and t
The principle of nucleic acid structure and recombination explains that hairpin formation, Holliday junction resolution and translation control ensure fidelity. Hence 1.0 μg/μl and 50 μg fits best because it matches molecular mechanisms of gene expression, as documented in authoritative sources on molecular biology.
Ref: Lodish et al., Molecular Cell Biology, 8th Edition, Chapter 8, RNA Processing and Translation, covers mRNA hairpins, puromycin mechanism and aminoacyl-tRNA function governing protein synthesis and translational regulation.
