Practice question
Question
The A ₂₆₀ of a plasmid solution after 100-fold dilution is 0.2. Given that A ₂₆₀ of 1.0 represents 50 μg/ml of DNA and the total volume of isolated plasmid solution is 50 μl, what will be the concentration and amount respectively of the isolated plasmid?
Explanation
The principle of nucleic acid structure and recombination explains that hairpin formation, Holliday junction resolution and translation control ensure fidelity. Hence 1.0 μg/μl and 50 μg fits best because it matches molecular mechanisms of gene expression, as documented in authoritative sources on molecular biology.
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