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#ZP3 glycoprotein

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Fertilization in mammals-l

Zona pellucida glycoprotein primarily binding sperm:

Species-specific sperm binding to human zona pellucida relies predominantly on recognition of intact ZP2 N-terminal domain within matrix context composed of ZP1-4 heteropolymers. Transgenic mouse models expressing human ZP genes show human sperm adhere only to zona containing human ZP2, sufficient alone to support binding, while human ZP3 insufficient. This contrasts mouse where ZP3 induces acrosome reaction. Following fertilization ovastacin cleaves ZP2 destroying binding epitope providing block. ZP1 crosslinks filaments structurally. Thus ZP2 provides primary ligand for human gamete attachme

Ref: Avella et al., J Cell Biol 2014: ZP2 as primary human sperm binding ligand in zona pellucida matrix.

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Fertilization in mammals-lll

ZP3 glycoprotein crucial for:

Mouse ZP3 serves as primary sperm receptor triggering acrosome reaction via aggregation of sperm surface receptors after binding O-linked glycans and protein epitopes. Clustering induces activation of heterotrimeric Gi proteins, phospholipase C, transient calcium and pH rise through CatSper and transient receptor potential channels, culminating in membrane fusion between plasma and outer acrosomal membranes and exocytosis of acrosomal contents including acrosin. This reaction exposes inner acrosomal membrane proteins necessary for secondary zona binding to ZP2 and for subsequent fusion. Capaci

Ref: Wassarman PM, Annu Rev Cell Biol 1987: ZP3 induced acrosome reaction signal transduction and calcium signaling.

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