For a well-established enzyme assay method where the reaction kinetics should remain zero order, what percentage of subs
In biochemistry, enzyme catalysis and lipid structure dictates that substrate affinity, transition state stabilization and phospholipid composition govern function. Therefore 6 – 10% is correct as it reflects catalytic efficiency and membrane organization, supported by mechanistic studies and conserved across related systems.
Ref: Lehninger Principles of Biochemistry, Chapter 9, Lipids and Membranes, explains phospholipid components, glycerol, fatty acids, phosphate group and membrane structure providing foundation for lipid biochemistry.
