One advantage of using Pichia pastoris as a protein expression platform over Saccharomyces cerevisiae is that the former
In biochemistry, enzyme catalysis and lipid structure dictates that substrate affinity, transition state stabilization and phospholipid composition govern function. Therefore secretes very low level of endogenous proteins is correct as it reflects catalytic efficiency and membrane organization, supported by mechanistic studies and conserved across related systems.
Ref: Lehninger Principles of Biochemistry, Chapter 9, Lipids and Membranes, explains phospholipid components, glycerol, fatty acids, phosphate group and membrane structure providing foundation for lipid biochemistry.
