The process of treadmilling in actin filaments is accelerated by:
Cellular actin turnover measured by fluorescence recovery after photobleaching half time 30 seconds versus minutes in vitro requires accelerated disassembly and reassembly. Treadmilling rate defined by addition at barbed end balanced by loss at pointed end, but spontaneous off rate modest. Accessory proteins increase both fluxes dramatically. Cofilin family severs ADP rich filament segments cooperatively, generating numerous short filaments exposing many pointed ends that depolymerize rapidly ten fold higher off rate. Cofilin also promotes debranching and filament twisting destabilizing contac
Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 17: Actin Treadmilling and Cofilin Profilin Regulation.
