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#Thermus aquaticus

2 public questions tagged with this topic.

PCR

PCR polymerase source Thermus aquaticus is

Thermus aquaticus was first isolated from high-temperature hot springs in Yellowstone National Park and grows optimally around 70-80°C. It thrives in such extreme thermal environments, classifying it as a thermophile, an extremophile adapted to sustain membrane integrity, enzymatic function, and protein stability under continuous thermal stress. Its DNA polymerase evolved to remain folded and catalytically active at near-boiling temperatures, resisting denaturation at 94-95°C used in PCR. Mesophiles grow at moderate temperatures, psychrophiles at cold, and halophiles in high salt, none supplyi

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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PCR

‘Taq’ polymerase utilized in PCR is a

Taq polymerase isolated from Thermus aquaticus functions as a DNA-dependent DNA polymerase. It uses single-stranded DNA as template and deoxyribonucleoside triphosphates as substrates to synthesize complementary DNA in the 5' to 3' direction, requiring a primer with free 3' hydroxyl group. It does not use RNA as template or synthesize RNA, distinguishing it from reverse transcriptases and RNA polymerases. Its exceptional thermostability allows high activity through denaturation cycles at 94°C, making iterative synthesis possible without fresh enzyme addition during polymerase chain reaction cy

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

View Details