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The non-coding strand (also called the template strand) serves as the template for RNA synthesis in transcription.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance Transcription - Mechanism and RNA Polymerase
DNA polymerase is the enzyme responsible for synthesizing new DNA strands during replication.
Ref: NCERT Class 11 Biology Chapter 9: Biomolecules Enzymes - Properties Classification and Cofactors
Despite double-stranded DNA chemically offering two complementary sequences, transcription uses only one as template to avoid production of overlapping complementary RNAs that would anneal into inert duplex. Within transcription bubble, polymerase binds template strand oriented 3' to 5' to synthesize RNA antiparallel, while coding non-template strand displaced. Template strand determination defined by promoter orientation and direction of polymerase binding. Only antisense strand read, also called template or transcribed strand, ensures mRNA sequence uniqueness. Using single strand prevents si
Ref: NCBI Biochemistry Transcription bubble template selection rationale; Lodish Chapter 8: One strand used as template mechanism
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