What type of support matrix is used in zone electrophoresis?
Zone electrophoresis separates molecules into discrete zones or bands on a stabilizing support to minimize convection and diffusion. Early methods employed filter paper or cellulose acetate strips saturated with buffer, providing a solid anticonvective medium. Samples migrate as discrete zones under applied voltage. Glass and plastic are not porous supports for zone stabilization. Modern zone electrophoresis uses agarose or polyacrylamide gels as improved supports, but historically and categorically, paper represents the classic support matrix distinguishing zone electrophoresis from moving-bo
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
