Sucrose is standard carbohydrate and energy source in plant tissue culture employed at 2 to 4 percent w/v, most commonly 30 grams per liter, because in vitro explants are predominantly heterotrophic due to limited photosynthetic capacity under low irradiance 40 micromoles per square meter per second and low CO2 in closed vessels. Plant cell wall invertases cleave sucrose into glucose and fructose which enter glycolysis generating ATP and NADPH through oxidative pentose phosphate pathway and provide carbon skeletons for amino acid, nucleotide, cell wall polysaccharide synthesis. Additionally su
Ref:
Calvin & Bassham photosynthesis; Plant Tissue Culture Bhojwani Chap. 2 sucrose role.