Skip to content
Login Register

#spindle formation

3 public questions tagged with this topic.

Cell Cycle Regulation

The centrosome cycle is coordinated by:

Centrosome duplication must occur once per cell cycle mirroring DNA replication to ensure formation of exactly two spindle poles. CDK2-Cyclin E in G1/S licenses disengagement of mother-daughter centrioles, while CDK1-Cyclin B, Polo-like kinase 1 and Aurora A coordinate maturation and separation later. Plk1 accumulates during G2, localizes to centrosomes via Polo-box binding to CEP192 phosphorylated by CDK1, and phosphorylates pericentrin, CEP215, NEDD9 enhancing recruitment of gamma-tubulin ring complexes and expanding pericentriolar material volume up to fivefold. Aurora A becomes active thro

Ref: Nigg & Stearns, Centrosome Cycle Coordinated by CDK1 Plk1 Aurora A, Nat Cell Biol 2011; Fu et al., Polo and Aurora Kinases Regulation.

View Details
Cell Cycle Regulation

Which kinase family plays a role in spindle formation and chromosome segregation?

Spindle construction requires cooperation between CDK1 and two mitotic kinase families distinct from CDKs. Aurora kinases A, B, C share conserved catalytic domains but differ in localization. Aurora A at centrosomes phosphorylates TACC3, ch-TOG, LATS2, CEP192 increasing pericentriolar material recruitment and microtubule nucleation. Aurora B as catalytic subunit of chromosomal passenger complex with INCENP, Survivin, Borealin resides at inner centromere until metaphase and midbody during cytokinesis. It phosphorylates Ndc80/Hec1, MCAK, Dam1/Ska complexes to destabilize attachments lacking tens

Ref: Nigg, Mitotic Kinases Aurora and Polo Regulation of Cell Division, Science 2001; Carmena et al., Aurora B in Error Correction.

View Details