Microculture is characterized by:
Microculture systems utilize miniaturized formats including 96-well, 384-well, and 1536-well plates with working volumes of 20-200 microliters. Characteristic feature is very low consumption of expensive reagents such as recombinant growth factors, fetal bovine serum, Matrigel, and small molecule libraries, making them economical for screening and optimization experiments. Environmental control is achieved via highly controlled incubators with plate hotels and automated liquid handling robots. Cells grow as small monolayers suitable for colorimetric assays like MTT for viability, fluorescence
Ref: Freshney Ch.8 Microculture low reagent use; Zhang et al Nat Rev Drug Discov 2005 High throughput screening in microplates.
