Which of the following techniques cannot be used to determine the molecular weight of a protein?
The principle of enzyme catalysis and lipid structure explains that substrate affinity, transition state stabilization and phospholipid composition govern function. Hence UV absorption fits best because it reflects catalytic efficiency and membrane organization, as documented in authoritative sources on biochemistry.
Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 7th Edition, Chapter 6, Enzyme Kinetics and Regulation, discusses Michaelis-Menten equation, transition state stabilization and allosteric control supporting enzyme function.
