Hybridoma cells survive in HAT medium because they:
Handling hybridoma cultures demands sensitive high-throughput detection secreted immunoglobulin microgram per mL among abundant serum proteins albumin bovine Ig. ELISA screening provides optimal format 96-well polystyrene plates coated purified antigen 1 ug/mL overnight 4 degrees passive hydrophobic adsorption van der Waals blocked 2 percent BSA 5 percent nonfat milk PBS preventing nonspecific binding plastic. Culture supernatant 50 uL added incubates 1h 37 degrees enabling specific binding Fab antigen interaction affinity association rate 10^5 per molar per second. After three washes PBS 0.05
Ref: Janeway Hybridoma Screening ELISA Technique Protocol; Alberts Protein A Purification Antibody Methods Chapter 8; Lodish Antibody Purification Immunotechniques Handbook Methods.