Retrohoming involves
Retrohoming describes mobility mechanism of Group II introns encoding reverse transcriptase and sometimes endonuclease in same polypeptide. Pathway initiates with intron RNA reverse splicing into single-stranded DNA target, then using target 3' end as primer, intron-encoded RT reverse-transcribes intron RNA into cDNA. Second strand synthesized by host repair polymerase. Thus retrohoming depends on reverse transcription converting RNA intermediate to DNA, producing permanent insertion. This retromobility inspired development of targetron gene editing biotechnology for site-specific gene disrupt
Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 6, Retrohoming of Group II introns via reverse transcription
