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Which one of the following is a fish cell line?

The principle of cellular compartmentalization and cell cycle explains that SNARE-mediated trafficking, Hayflick limit, P53 and cyclin regulation control proliferation. Hence RTG -2 fits best because it aligns with membrane trafficking and cell cycle control, as documented in authoritative sources on cell biology.

Ref: Alberts et al., Molecular Biology of the Cell, 6th Edition, Chapter 17, Cell Cycle and Regulation, explains Cdc2, cyclins, P53, proteasomes, Hayflick limit, primary culture and immortalization mechanisms governing proliferation.