Electrophoresis of a purified protein in SDS-PAGE in the presence of 2- mercaptoethanol yields two bands of 35 kDa and 4
Mechanistically, enzyme catalysis and lipid structure involves substrate affinity, transition state stabilization and phospholipid composition govern function. This validates The protein is a heterodimer because it reflects catalytic efficiency and membrane organization, a pattern repeatedly demonstrated in biochemistry research.
Ref: Lehninger Principles of Biochemistry, Chapter 9, Lipids and Membranes, explains phospholipid components, glycerol, fatty acids, phosphate group and membrane structure providing foundation for lipid biochemistry.
