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#Ras protein

5 public questions tagged with this topic.

Lipid Link Protein

What is the function of Ras protein?

Ras family H-Ras, K-Ras, N-Ras are small monomeric GTPases of 21 kDa acting as molecular switches downstream of receptor tyrosine kinases and G-protein coupled receptors to regulate cell proliferation, differentiation, survival, cytoskeletal organization and metabolism. Cycle between GDP-bound inactive and GTP-bound active conformations controlled by guanine exchange factors SOS that promote GTP loading upon growth factor stimulation and GTPase activating proteins p120GAP that accelerate intrinsic GTP hydrolysis turning signal off. Active Ras-GTP recruits effectors Raf kinases activating MEK-E

Ref: Cox & Der, Nat Rev Drug Discov 2010, Ras signaling; Prior et al., Cancer Res 2012.

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Lipid Link Protein

Which lipid anchor type is involved in signal transduction and is found in Ras protein?

Ras small GTPases H-Ras, N-Ras, K-Ras are central hubs in growth factor signaling linking receptor tyrosine kinases to Raf-MEK-ERK MAPK cascade regulating proliferation, differentiation, survival and cytoskeletal remodeling. For signaling competence Ras must associate with inner leaflet of plasma membrane where it interacts with effectors. This localization depends on C-terminal CAAX prenylation. H-Ras, N-Ras, K-Ras4A undergo farnesylation of CAAX cysteine by farnesyltransferase forming thioether linked 15-carbon anchor, followed by RCE1 cleavage and ICMT methylation. Additional second signal

Ref: Hancock, Nat Rev Mol Cell Biol 2003, Ras membrane targeting; Prior et al., Cancer Res 2012.

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Lipid Link Protein

Which statement is correct about farnesylation?

Farnesylation is post-translational prenylation catalyzed by heterodimeric farnesyltransferase FTase using farnesyl pyrophosphate derived from mevalonate cholesterol biosynthesis pathway as donor. Modification occurs at CAAX motif at extreme C-terminus where C is cysteine, A aliphatic residues, X typically methionine, serine or glutamine directing farnesyl addition versus leucine directing geranylgeranyl addition. Reaction forms thioether bond between cysteine sulfur and C1 of isoprenoid. Farnesyl group is linear 15-carbon isoprene polymer comprising three isoprene units with 3 double bonds pr

Ref: Zhang & Casey, Annu Rev Biochem 1996, Farnesylation; Wang & Casey 2016.

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Cell Signaling ELR Pathways - I

Which step converts Ras from inactive to active form?

GDP–GTP exchange, is consistent with established principles of cell signaling, receptor pharmacology and cellular regulation. Experimental measurements of binding parameters, genetic loss-of-function studies and pharmacological interventions all converge on the same interpretation. Related options address neighboring concepts but do not satisfy the precise criterion stated in the question.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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Cell Signaling Fundamental PrinciplesI

Ras is active when bound to

GTP, is consistent with established principles of cell signaling, receptor pharmacology and cellular regulation. Experimental measurements of binding parameters, genetic loss-of-function studies and pharmacological interventions all converge on the same interpretation. Related options address neighboring concepts but do not satisfy the precise criterion stated in the question.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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