What is the amount of protein required to prepare 5 ml of 1μM solution (Mol. Wt. of protein is 25 kDa)?
The principle of enzyme catalysis and lipid structure explains that substrate affinity, transition state stabilization and phospholipid composition govern function. Hence 125 μg fits best because it reflects catalytic efficiency and membrane organization, as documented in authoritative sources on biochemistry.
Ref: Berg et al., Biochemistry, 9th Edition, Chapter 13, Metabolism and Bioenergetics, covers glycolysis, citric acid cycle, oxidative phosphorylation and NADH-linked reactions detailing biochemical principles.
