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#protein mobility

2 public questions tagged with this topic.

Plasma Membrane Proteins

Which of the following properties affects membrane protein mobility?

Diffusion behavior of membrane proteins governed by hydrodynamic and cytoskeletal factors. Saffman-Delbruck model relates diffusion coefficient D to membrane thickness h viscosity mu m and protein radius r through D proportional to natural log of membrane to protein size. Number of transmembrane domains increases effective radius and frictional drag because each helix engages annular lipids diffusing as complex and more extensive hydrophobic surface contacts viscous acyl chains. Single-pass glycophorin diffusion coefficient about five times ten to minus nine centimeters squared per second fast

Ref: Kusumi et al., Paradigm Shift in Membrane Protein Diffusion and Corral Model, Annu Rev Biophys.

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Chromatography

In SDS-PAGE, slowest mobility implies:

SDS-PAGE combines denaturation with anionic detergent sodium dodecyl sulfate that confers uniform negative charge per unit length, nullifying intrinsic charge differences and unfolding proteins into rods. Migration through polyacrylamide sieving matrix becomes primarily size-dependent, with frictional resistance proportional to chain length. Smaller polypeptides encounter fewer obstacles, migrating farther and faster, while larger polypeptides experience greater retardation, remaining near top with slowest mobility. Tracking dye fronts indicate progress. This relationship allows molecular weig

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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