You are interested in a particular enzyme that is expressed in various human tissues. You have isolated the protein from
The principle of enzyme catalysis and lipid structure explains that substrate affinity, transition state stabilization and phospholipid composition govern function. Hence TBLASTN using the protein sequence as query and the human genome sequence as ... fits best because it reflects catalytic efficiency and membrane organization, as documented in authoritative sources
Ref: Berg et al., Biochemistry, 9th Edition, Chapter 13, Metabolism and Bioenergetics, covers glycolysis, citric acid cycle, oxidative phosphorylation and NADH-linked reactions detailing biochemical principles.
