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#protein comparison

2 public questions tagged with this topic.

Transgenic Plant -1

Vip proteins differ from Cry proteins because they are:

Cry proteins form parasporal crystals during sporulation, requiring solubilization at high pH and proteolytic activation. Vip proteins differ temporally and biochemically. Vip1, Vip2, Vip3 secreted during exponential vegetative growth into culture supernatant, not associated with spores. Vip3A, most agriculturally relevant, shares no sequence homology with Cry domains, presents unique tetrameric structure and binds distinct receptors including scavenger receptor class C and fibroblast growth factor receptor-like protein, triggering apoptosis pathway in addition to pore formation. Activity prim

Ref: Estruch PNAS 1996 Vip vegetative; Donovan 2001 Vip3 structure; Yu Appl Environ Microbiol 1997 differences; PubMed 8855281 vegetative insecticidal proteins.

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Cytoskeleton

The actin cytoskeleton in prokaryotic cells is functionally similar to which of the following eukaryotic proteins?

Prokaryotic cells maintain rod shape and organize cell wall synthesis without eukaryotic compartments using cytoskeletal homologs sharing ancient ATPase fold. MreB, belonging to actin Hsp70 superfamily, shares structural core of five conserved sequence motifs forming ATP binding pocket between domains IA and IIA. Purified MreB polymerizes into ATP dependent antiparallel double filaments that rotate circumferentially beneath cytoplasmic membrane driven by cell wall elongation machinery. It scaffolds elongasome complex containing RodA glycosyltransferase, PBP2 transpeptidase and MreC MreD, direc

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 16: Cytoskeleton and Bacterial Homologs MreB.

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