Introduction of a non-sense mutation in a eukaryotic protein coding gene often leads to
metabolic engineering and inheritance analysis shows exogenous gene introduction and pathway extension enable trait improvement. Consequently increased degradation of the mRNA in the nucleus cytoplasm emerges as the valid choice since it demonstrates metabolic extension and combinatorial biosynthesis, aligning with established principles in genetics and biotechnology literature.
Ref: Griffiths et al., Introduction to Genetic Analysis, 11th Edition, Chapter 5, discusses metabolic pathways, recombinant DNA technology, metabolic extension and Golden Rice as pathway engineering for vitamin A production.
