Which one of the following techniques will help to overcome a pre fertilization barrier between the two species?
The principle of genetic engineering explains that vector design and pathway extension enable vitamin production. Hence Protoplast fusion fits best because it utilizes recombinant DNA and metabolic engineering, as documented in authoritative sources on biotechnology.
Ref: Primrose and Twyman, Principles of Gene Manipulation and Genomics, 8th Edition, Chapter 2, Recombinant DNA Technology, discusses Golden Rice development, exogenous genes, metabolic engineering and vitamin A pathway extension supporting applications.
