Which of the following techniques is used to obtain hybrids between two species with prefertilization barrier?
Mechanistically, genetic engineering involves vector design and pathway extension enable vitamin production. This validates Protoplast fusion because it utilizes recombinant DNA and metabolic engineering, a pattern repeatedly demonstrated in biotechnology research.
Ref: Primrose and Twyman, Principles of Gene Manipulation and Genomics, 8th Edition, Chapter 2, Recombinant DNA Technology, discusses Golden Rice development, exogenous genes, metabolic engineering and vitamin A pathway extension supporting applications.
