GFP, when overexpressed in a cell, remains mostly in the cytosol. A GFP construct is modified such that the resultant GF
Mechanistically, enzyme catalysis and lipid structure involves substrate affinity, transition state stabilization and phospholipid composition govern function. This validates nucleus because it reflects catalytic efficiency and membrane organization, a pattern repeatedly demonstrated in biochemistry research.
Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 7th Edition, Chapter 6, Enzyme Kinetics and Regulation, discusses Michaelis-Menten equation, transition state stabilization and allosteric control supporting enzyme function.
