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#nucleic acid enzymes

2 public questions tagged with this topic.

Biochemistry and Aptitude and Reasoning

Which enzyme is used to remove the phosphate group from 5' end of the DNA?

Mechanistically, enzyme catalysis and lipid structure involves substrate affinity, transition state stabilization and phospholipid composition govern function. This validates Alkaline phosphatase because it reflects catalytic efficiency and membrane organization, a pattern repeatedly demonstrated in biochemistry research.

Ref: Lehninger Principles of Biochemistry, Chapter 9, Lipids and Membranes, explains phospholipid components, glycerol, fatty acids, phosphate group and membrane structure providing foundation for lipid biochemistry.

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Nucleic acid probe and labelling

Which enzyme is used in 3’-end labeling of nucleic acids?

3'-end labeling adds labeled nucleotides specifically to the free 3'-hydroxyl terminus of nucleic acids. Terminal deoxynucleotidyl transferase is a template-independent DNA polymerase that catalyzes sequential addition of deoxynucleotides to 3' ends without requiring duplex formation. Using substrates like [alpha-32P]dideoxyATP, [alpha-32P]cordycepin, or biotin-dUTP, it creates singly or multiply tailed labeled probes. This enzyme efficiently labels single-stranded DNA, double-stranded DNA, and RNA primers. Klenow fragment requires a template and primer, Taq polymerase needs thermal cycling an

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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