A synthetic mRNA of repeating sequence 5'- CACACACACACACACAC... is used for a cell-free protein synthesizing system like
Mechanistically, nucleic acid structure and recombination involves hairpin formation, Holliday junction resolution and translation control ensure fidelity. This validates one protein, with an alternating sequence of two different amino acids because it matches molecular mechanisms, a pattern repeatedly demonstrated in molecular biology research.
Ref: Lodish et al., Molecular Cell Biology, 8th Edition, Chapter 8, RNA Processing and Translation, covers mRNA hairpins, puromycin mechanism and aminoacyl-tRNA function governing protein synthesis.
