Which of the following is a neoschizomer pair?
Neoschizomers are restriction enzymes that recognize identical or closely related DNA sequences but cleave at different phosphodiester bonds, producing distinct termini. Isoschizomers cut identically, while neoschizomers illustrate variation in cleavage position. XhoI recognizing C^TCGAG and SalI recognizing G^TCGAC are classic textbook examples frequently grouped as neoschizomers because they recognize overlapping TCGA core motifs and generate compatible cohesive ends with different overall specificity, demonstrating same-site family with divergent cut patterns. Understanding this distinction
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
