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#mRNA localization

2 public questions tagged with this topic.

Which of the following refers to regionally localized proteins or mRNAs in an unfertilized egg that regulate development

Unfertilized eggs establish developmental asymmetry through regionally localized cytoplasmic determinants composed of maternal mRNAs, regulatory proteins and organelles tethered to the cytoskeleton at specific cortical domains. Examples include bicoid mRNA anchored anteriorly in Drosophila oocytes and VegT and Vg1 localized vegetally in Xenopus. Upon fertilization and cleavage, daughter blastomeres inherit different quantities of these factors, which activate selective transcription factor cascades before zygotic genome activation. This prelocalized information underlies autonomous specification, establishing body axes, germ layers and cell polarity without requiring immediate cell-cell signaling or external gradients.

Ref: Gilbert, Developmental Biology, 12th ed., Chapter 2: Cytoplasmic Determinants and Maternal Effect Genes.

Gurken mRNA localization specifies:

Gurken mRNA localizes to oocyte nucleus corner near anterodorsal follicle cells translating into EGF-like ligand activating Torpedo receptor dorsally, initiating dorsal follicle fate via repression of Pipe sulfotransferase. Ventral follicle cells retain Pipe modifying vitelline membrane to permit ventral Spatzle processing activating Toll and Dorsal gradient. Cascade specifies DV axis of eggshell with dorsal appendages and embryo with ventral mesoderm. Gurken transport driven by dynein and localized translation is symmetry-breaking event upstream of eggshell patterning, not specifying anteroposterior, left-right, or segmentation patterns dependent on bicoid, nodal, or pair-rule systems.

Ref: Gilbert, Developmental Biology, 12th ed., Chapter 9: Gurken mRNA localization specifies DV axis via EGFR Torpedo signaling.