MRC-5 cells are derived from:
Death phase also called decline phase is final segment of in vitro growth curve where net cell number decreases as death rate surpasses division rate. Triggered by exhaustion of essential nutrients glucose and L-glutamine, severe acidification due to lactic acid production, accumulation of toxic ammonium from glutaminolysis, oxidative stress from reactive oxygen species generated by mitochondrial dysfunction, and activation of intrinsic apoptosis pathway involving Bax translocation, cytochrome c release from mitochondria, apoptosome formation with Apaf-1, and executioner caspase-3 cleavage of
Ref: Freshney Ch.13 Death phase decline apoptosis; Elmore S Toxicol Pathol 2007 Apoptosis intrinsic pathway caspase activation.
