What happens to microtubule-based transport if dynein function is inhibited?
Intracellular transport operates through antagonistic plus-end kinesins and minus-end dynein along same microtubule tracks. Under normal conditions kinesin-1 and kinesin-3 carry cargo outward toward cell periphery, dynein-dynactin returns cargo toward centrosome where microtubule minus ends converge. Acute inhibition by overexpression of dynamitin CC1 fragment dissociating dynactin, treatment with ciliobrevin D blocking AAA1 ATPase, or inducible degron of dynein heavy chain abolishes retrograde motion while anterograde motion persists. Consequences include dispersion of Golgi, accumulation of
Ref: Schroer Annu Rev Cell Biol; dynein inhibition causes peripheral cargo accumulation, Golgi dispersion.
