Which domain of dynein contains the microtubule-binding region?
Dynein heavy chain organization subdivided into distinct functional modules arranged linearly from N to C terminus: tail stem region for cargo adaptor binding and dimerization via intermediate chain interactions, motor ring core, and stalk linker elements bridging. Tail contains dimerization domain plus binding sites for light intermediate chains controlling cargo selectivity not microtubule binding. Central motor ring of six AAA domains AAA1 to AAA6 forming closed asymmetric ring plus protruding antiparallel coiled coil stalk emerging between AAA4 small and large subdomains after second coile
Ref: Gibbons et al., J Cell Biol 2005; Carter 2013 – Dynein stalk domain contains microtubule-binding region.
