Skip to content
Login Register

#metabolic activity

3 public questions tagged with this topic.

Animal Tissue Culture 3

MTT assay measures:

MTT assay developed by Tim Mosmann in 1983 measures cell viability and proliferation based on reduction capacity of living cells. Principle uses yellow tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide which enters cells via endocytosis and is reduced by mitochondrial succinate dehydrogenase of complex II and cytosolic NAD(P)H-dependent oxidoreductases cleaving tetrazolium ring with electrons from NADH and NADPH producing insoluble purple formazan crystals accumulating intracellularly in mitochondria and lipid droplets. Metabolically incompetent dead cells fail to r

Ref: Mosmann T J Immunol Methods 1983 MTT viability assay; ATCC MTT Cell Proliferation Assay mitochondrial dehydrogenase reduction protocol.

View Details
Cell Division

Which phase of the cell cycle is the most metabolically active?

Cellular metabolism is tightly coupled to cell cycle progression, with G1 phase exhibiting maximal biosynthetic activity to support doubling of mass before DNA replication. Upon growth factor binding, PI3K-Akt-mTORC1 pathway stimulates glucose uptake through GLUT1, increases glycolytic flux and pentose phosphate pathway for nucleotide precursors, enhances mitochondrial biogenesis via PGC1-alpha and mitochondrial transcription factor TFAM, and activates ribosome biogenesis through RNA polymerase I mediated rDNA transcription and S6K phosphorylation of ribosomal protein S6. Translation initiatio

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 17: G1 Metabolism and Growth.

View Details
Electrophysiological methods

Which brain imaging method measures metabolic activity using positron emission?

Positron emission tomography images metabolic activity by injecting tracer molecules labeled with short-lived positron emitters such as fluorine-18, carbon-11 or oxygen-15. Inside tissue, emitted positron travels briefly then annihilates with an electron, producing two 511 keV gamma photons emitted in opposite directions. Ring detectors register coincident photon pairs, reconstructing tracer concentration. Fluordeoxyglucose PET highlights brain regions with high glucose utilization, reflecting synaptic activity. MRI visualizes anatomy via proton magnetic resonance, CT uses X-ray attenuation, S

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

View Details