3 public questions tagged with this topic.
From the spectrochemical series, F− is the weakest field ligand.
Ref: NCERT Class 12 Chemistry > Chapter 5: Coordination Compounds > Topic: Crystal Field Theory - CFT and Crystal Field Splitting and CFSE
[Co(en)3]3+ contains 3 ethane-1,2-diamine (en) ligands, a bidentate ligand denoted as H₂NCH₂CH₂NH₂.
Ref: NCERT Class 12 Chemistry > Chapter 5: Coordination Compounds > Topic: Colour and Magnetic Properties and Stability and Importance
In Surface Plasmon Resonance terminology, ligand refers to the molecule permanently attached to the sensor chip dextran matrix, while analyte is the soluble partner that flows in microfluidic channels. Immobilization is typically achieved via amine, thiol, or streptavidin coupling, ensuring stable baseline and reproducible orientation. Ligand density influences response magnitude and kinetic accessibility. Analyte binding to ligand produces resonance unit increase. Distinguishing ligand and analyte is crucial for kinetic modeling, since immobilization restricts diffusion and may affect binding activity. Antibody coating or bead systems are not used in conventional SPR sensor design.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
