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#kanamycin resistance

4 public questions tagged with this topic.

Aptitude and Reasoning

Transgenic plants were developed with a gene conferring krummycin resistance. Four independent Te events were seeted and

quantitative reasoning analysis shows ratio, percentage and series logic determine correct calculation. Consequently Line Two emerges as the valid choice since it meets mathematical and logical criteria, aligning with established principles in aptitude and reasoning literature.

Ref: Agrawal, Quantitative Aptitude for Competitive Examinations, Chapter 5, Logical Reasoning and Probability, covers ratios, percentages, series completion, time-distance and analytical reasoning explaining problem-solving strategies for competitive assessments.

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Microbiology and Physiology

nptII gene imparts resistance to

Mechanistically, metabolic engineering and inheritance involves exogenous gene introduction and pathway extension enable trait improvement. This validates Kanamycin because it demonstrates metabolic extension and combinatorial biosynthesis, a pattern repeatedly demonstrated in genetics and biotechnology research.

Ref: Pierce, Genetics: A Conceptual Approach, 6th Edition, Chapter 3, discusses genotype-phenotype correlation and inheritance patterns providing framework for genetic transmission and metabolic engineering.

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Gene Cloning Process

Which selectable marker is associated with kanamycin resistance?

Kanamycin resistance in cloning vectors is generally mediated by kanamycin resistance determinant encoding aminoglycoside 3'-phosphotransferase type II, product of nptII or kanR gene derived from bacterial transposon Tn5. This enzyme phosphorylates kanamycin, inactivating its ability to bind 30S ribosomal subunit and cause mistranslation and bacterial cell death. Cells expressing the resistance grow on media containing kanamycin at 25-50 µg per mL for selection. Neomycin resistance similarly uses nptII, while AmpR encodes β-lactamase and TetR encodes tetracycline efflux pump with distinct anti

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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